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KMID : 0371619930090010041
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Journal of Wonkwang Medical Science
1993 Volume.9 No. 1 p.41 ~ p.54
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An In Vitro Study on the Cytotoxicity of Clomiphene Citrate in Mouse Ova and Early Embryos
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전혜경
반승일/문연자/박승택/정연태
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Abstract
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In order to elucidate the cytotoxicity of clomiphene citrate, the rate of in vitro fertilization(IVF) of ova of the developmental rate of early embroys in A-strain mouse were examined.
The rate of ova cleavaging to 2-cell stage by IVF was remarkably decreased(12.6%) at the concentration of 10㎕/㎖ of clomiphene than that of the control(65.2%) when the ova fertilized in vitro were cultured for 24 hours in the untreated medium
after
treatment ova with clomiphene for 5hrs, But there was no ovum cleavaging to 2-cell stage at 50㎕/㎖ clomiphene except only a few 2-polar bodied ova.
The developmental rate of mouse early embryos was decreased dose-dependently. Especially at 25㎕/㎖ of clomiphene, the number embryos cleavaging to blastocyst from 2-cell stage(41.4%) was decreased remarkably compared with that of the
control(87.5%).
And only a few embryos were developed to 8-cell stage at clomiphene concentration of 50㎕/㎖ after 2-cell embryos were incubated for 72 hours in clomiphene-treated medium.
Morphological changes such as fragmentation and fusion of blastomeres, cytolysis and developmental retardation were increased with dose-dependently.
These results suggest that clomiphene has cytotoxic effect by the decrease of the rate of IVF, the developmental rate and the degenerative changes of murine ova and early embryos.
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